Bacteriology and Infectious Diseases

Biography

Biography: Whika Febria Dewatisari

Abstract

Sansevieria trifasciata used as an ornamental plant. It also used as a traditional medicine for influenza, cough and inflammation of the respiratory tract. The roots and leaves of S. trifasciata contains many secondary metabolites such as saponins that efficacious as a cough remedy to treat sprains, injuries hit, venomous snake bites, ulcers, cough, inflammation of the respiratory tract and hair growth. Microbes are used to seeing the antibacterial saponins activities of S. trifasciata are Escherichia coli and Staphylococcus aureus. The main reason for used these microbes because E. coli is a bacterium that causes diarrhea and S. aureus is one of the bacteria that cause cough in humans. Plants were used S. trifasciata var. Golden Hahnii. Based on research by Dewatisari (2008), states that the variety had the highest saponin content of S. trifasciata is Golden Hahnii among other varieties and the parts that had the highest saponin were in its roots. This article aims to explain quantitatively the effectiveness of saponin compounds from the roots of S. trifasciata var. Golden Hahnii as anti-microbials in inhibiting the growth of bacteria S. aureus and E. coli. Methods of data collection were sample preparation, a preliminary test, the extraction of saponins, antibacterial activity test and isolate compound separation by TLC (Thin layer Chromatography). This study includes the extraction of the active compounds in the roots of S. trifasciata by maceration with methanol 90%. Separation of the active compound was conducted by TLC. Eluent used was chloroform; methanol; water with various concentration (13: 4: 1), (65; 50: 10), (20: 60: 4), 20: 60: 10). Antibacterial test conducted by the disc diffusion method against S. aureus and E. coli. Identification of test compounds triterpenoid saponins using foam and Liebermann-Burchard color test (LB). The results showed that the extract of the roots has potential as anti-bacterial. The extract was able to inhibit the growth of E. coli and S. aureus. At the optimum concentration of 200 ppm produced inhibition zone was 26.5 mm to 20.2 mm for S. aureus and E. coli. Best eluent to separate the triterpenoid saponins in root extracts of S. tifasciata was chloroform; methanol; water at a concentration (20: 60: 4) with 3 separate visible stains are: 0.135; 0.85; 0.815. Mechanism root extract as an anti-bacterial S. trifasciata was synergistic. It was seen from the inhibition zone, for E. coli isolates I=5.52 mm and isolates II=2.50 mm, for S. aureus isolates I=1.52 mm and isolates II=0.58 mm while the isolates III is not effective as an anti-bacterial.