Nadia Mukhtar
University of Veterinary & Animal Sciences, Paksitan
Title: Antimicrobial Resistance Genes In Salmonella Isolates From Poultry Drinking Water
Biography
Biography: Nadia Mukhtar
Abstract
Salmonella species are among the most common causes of human bacterial gastroenteritis worldwide and food animals specially poultry are important reservoirs of this bacteria. In recent years, due to increase in the occurrence of antimicrobial resistance spp. of Salmonella, fatality rate for Salmonellosis is increasing significantly. In the context of exploring the emergence of antimicrobial resistant Salmonella species, present study was planned. Salmonella isolation was performed by collecting samples from poultry rearing and slaughtering areas with recovery rate about 29.3 %. Bacterial colonies of red color with black center were appeared on the XLD agar plates and then confirmed by biochemical tests. Then multi-drug resistance of the isolates was examined by disk diffusion method. Resistant samples were further analyzed for the detection of various tet genes (tetA, tetB, tetC, tetD, tetG). PCR confirmed the presence of tetA in all the Salmonella positive samples while tetB was present in combination with tetA gene only in 16 samples. No amplification of tetC, tetD and tetG was examined. Our results illustrate that commonly used antibiotics, especially Tetracycline is showing decline in efficacy due to increasing antimicrobial resistance in locally isolated Salmonella from poultry samples. These findings indicate that local population of Salmonellacontains the tetA alone or in combination with tetB gene and are likely played an important role in transmission of antimicrobial resistance determinants among Salmonella strains.