Bacteriology and Infectious Diseases

Biography

Biography: Xuejun Jingyue

Abstract

Aims: To demonstrate the antimicrobial resistance phenotypes and genotypes of a multi-drug resistant Klebsiella pneumoniae isolate recovered from Giant Panda ( Ailuropoda melanoleuca ). Methods and Results: In December 2015, we obtained a vaginal swab sample from a Giant Panda named Huajiao (3 years old) lived in China Conservation and Research Center for the Giant Panda. Drug-resistant bacteria were isolated from Brain-Heart infusion agar containing cefotaxime (8 µg/mL). Biochemical characterization and antimicrobial testing were conducted using the BD Phoenix 100 automated microbiology system. All isolates were screened for the presence of antimicrobial resistance genes and analysed for genetic relatedness by multiplex PCR assays. Conjugation experiment was carried out in broth using azide-resistant E. coli strain J53 as the recipient. A ST1068-MDR-Klebsiella pneumoniae was isolated from the vaginal swab sample, the isolate was resistant to Amikacin, Gentamicin, Imipenem, Meropenem, Cefazolin, Ceftazidime, Cefotaxime, Cefepime, Aztreonam, Ampicillin, Piperacillin, Amoxicillin-Clavulanate, Piperacillin-Tazobactam, Trimethoprim-Sulfamethoxazole, Chloramphenicol, the antimicrobial resistance genes blaCTX-M-15, blaNDM-1, armA and sul1 were detected. Resistance genes blaCTX-M-15 and blaNDM-1 were carried by two different plasmids, the blaCTX-M-15-carrying plasmid was transferred by conjugation and was classified as IncA/C, the blaNDM-1-carrying plasmid was also transferred but haven’t been classified. Conclusions: This study alarmed that blaNDM-1 gene have intruded into the ecosystem of giant panda up to now, the ST1068-MDR-Klebsiella pneumoniae strain co-producing blaCTX-M-15, blaNDM-1, armA and sul1 genes was the first report, it' s uncertain where was it from. In this canse enterobacteria serve as a critical vector in spreading antimicrobial resistance in the ecosystem of Giant Pandas.