Bacteriology and Infectious Diseases

Biography

Biography: Habimana Bosco

Abstract

The diagnosis of Tuberculosis in development country remain a big issue where we are trying to Introduce new diagnosis in order to control the diseases , such as the Gene xpert system and Led Microscopy have been described as promising techniques for rapid diagnosis of tuberculosis but lack of sensitivity of Led microscope compare to Molecular diagnostic , With the aim of evaluating the specifity and sensitivity of Led microscopy with Genexpert method on samples of spatum of patients suspected of tuberculosis we analyzed 267 clinical samples, using as a Led microscopy Using Auramine staining and molecular diagnosis( Genexpert ) with dectecte of M. tuberculosis genome and Rifampicine detection . Molecular diagnosis (Gene Xpert method showed higher sensitivity in patients with Pulmonary tuberculosis (Where some cases missing by auramine staining are shown by Genexpert We analyzed sputum from 267 patients in the different age suspected of TB, admitted in the period from October 2015 to 1 ST November 2015 at the outpatient clinic at GISENYI Hospital , when compared to patients with the Tuberculosis using Auramine staning Vs Genexpert in 267 Patient we see that 15 patient are find with pulmonary tuberculosis among this patient one are reported as MultDrugResistant cases . Comparing the results of auramine staning using Led Microscopy for detection of M. tuberculosis in spatum we see all missing 5 cases auramine staining will be showed by molecular diagnosis by using Genexpert . the xpert showed higher sensitivity compare to Led microscopy using auramine staining Genexpert is a rapid technique that, it showing a good sensitivity, The performance of Genexpert techniques should be considered and tested in future works on specimens like sputum, readily obtainable in most cases. The improving of M. tuberculosis Using Genexpert and Rif detection is very sensitive, Also, TB smear microscopy is highly insensitive for HIV- co infected individuals and for children due to the reduced pulmonary bacillary loads in these patients. TB affected patients will give the possibility of an earlier detection of bacilli thus interrupting the transmission chain of the disease. Keywords: Mycobacterium tuberculosis, Genexpert method , sputum ,molecular diagnosis, Led microscopy (auramine staining ).Multidrug resistance.