Maysaa Mohammedawi
AL Nahrain University, Iraq
Title: Molecular Study on Streptococcus pneumoniae Isolated from Patients Suffering from Respiratory Tract Infection Using RAPD-PCR Technique
Biography
Biography: Maysaa Mohammedawi
Abstract
A total of 53 sputum samples were collected from patients suffering from respiratory tract infections attending different hospitals of Baghdad and Al-Anbar (Ramadi) governorates. From these samples only fifty bacterial isolates were obtained and identified. According to the morphological and cultural characteristics results showed that 14 bacterial isolates (28%) were belong to Streptocoocus spp., 26 bacterial isolates (52%) were belong to Staphylococcus spp., four bacterial isolates (8%) were pseudomonas spp., and six isolates (12%) were identified as Klebsiella spp. Then further steps have been proceeded for identification Streptocoocus isolates species by biochemical tests, API 20 STEP system and blood hemolysis patterns. Results of full identification showed that only three isolates (21.4%) were identified as Streptococcus pneumoniae, five isolates (35.7%) were Streptococcus pyogenes, two isolates (14.3%) were Streptococcus mutans, two isolates (14.3%) were Streptococcus sangarius, one isolate (7.1%) was Streptococcus fecalis, and one isolate (7.1%) was Streptococcus agalacticae. Results of the antimicrobial susceptibility showed that these isolates gave different patterns of sensitivity to different antibiotics. For molecular diagnosis of these S. pneumoniae isolates and to study the epidemiology source of these isolates, ten different oligonucleotide primers were used and results showed that the genetic similarity was 67.92% between N8 and N6, while it was found to be 52.27% and 53.76% between N11 and N6 and N11 and N8, respectively. The source of isolation of three isolates confirms these results because the isolates N6 and N8 were isolated from Baghdad governorate while the isolate N11 was isolated from Al-Ramadi city (Al-Anbar province).